นพ. ตนุพล จบการศึกษาจากแพทยศาสตร์บัณฑิตจากคณะแพทยศาสตร์ โรงพยาบาลศิริราช หลังจากนั้น ได้ศึกษาต่อทางด้านการดูแลป้องกันและฟื้นฟูสุขภาพ และได้รับวุฒิบัตร American Board of Anti-Aging and Regenerative Medicine (ABAARM) จากชิคาโก ประเทศสหรัฐอเมริกา

• Fellowship in Anti-Aging, Metabolic and Functional Medicine (FAAMFM), American Academy of Anti-Aging Medicine, Chicago, Illinois, USA
• Doctor of Medicine, Faculty of Medicine at Siriraj Hospital, Mahidol University, Thailand
• American Board of Anti-Aging and Regenerative Medicine (ABAARM), American Academy of Anti-Aging Medicine, Chicago, Illinois, USA
• American College of Anti-Aging Sports Medicine Professionals (ACASP), American Academy of Anti-Aging Medicine, Chicago (A4M), Chicago, Illinois, USA
• Fellowship in Anti-Aging and Regenerative Medicine (FAARM), American Academy of Anti-Aging Medicine, Chicago, Illinois, USA
• Fellow of the European Committee of Sexual Medicine (FECSM), Amsterdam, Netherlands
• Fellowship in Stem Cell Medicine, American Academy of Anti-Aging Medicine (FSTCM), Chicago Illinois, USA
• Fellowship in Obesity Medicine/ American Society of Bariatric Physicians (ASBP), Denver Colorado, USA
• Thai Board of Preventive Medicine (Public Health), The Medical Council of Thailand
• Preventive Medicine Association of Thailand, The Medical Council of Thailand

• Certificated of The Oxford Strategic Innovation Programme, Saïd Business School, University of Oxford, England
• Certificated of The International Board of Lifestyle Medicine (IBLM), Diplomate of American College of Lifestyle Medicine Physician, Oregon, USA
• Certificated of Covid-19 Contact Tracing Course, Johns Hopkins University, Maryland, USA
• Certificated of Music For Wellness, Berklee College of Music Program, Boston, USA
• Certificated of Digital Disruption: Digital Transformation Strategies Course, University of Cambridge, England
• The 27th Mini Master of Management in Health (Mini M.M. in Health), ASEAN Institute for Health Development(AIHD), Mahidol University and Thailand Preventive Medicine, Thailand
• Certificated of Managing in Healthcare Delivery, Harvard Business School, Harvard University, Boston USA
• Specialist Certification of Obesity Professional Education (SCOPE), World Obesity Federation, London, UK
• Certificated of Advance training in Obesity Medicine, American Board of Obesity Medicine (ABOM), Denver Colorado, USA
• Certificated of the European school of Sexual Medicine, European Society of Sexual Medicine (ESSM), Oxford, United Kingdom
• Certificated of Advance Training in Bariatric Medicine (ATBM), American Board of Bariatric Medicine (ABBM), Denver, Colorado, USA
• Certificated of Advance Training in Pluripotent Stem Cell Technology, Oxford University, United Kingdom
• Certificated of Cannabinoid Medicine Curse, Occupational and Environmental Medicine Association of Thailand, Thailand